The sensitive analytical method known as high-performance liquid chromatography (HPLC) is frequently employed in the separation and examination of glycoproteins and the carbohydrates that they are formed from. Creative Biolabs is dedicated to provide a comprehensive range of glycoprotein analysis services to clients worldwide, based on this methodology.

HPLC-Based Glycoprotein Analysis

A sample combination or analyte is pumped down a column containing chromatographic packing material (stationary phase) at high pressure using a solvent known as the mobile phase in high-performance liquid chromatography (HPLC). A stream of flowing nitrogen or helium carrier gas carries the sample.

Any substance that can dissolve in a liquid and have trace amounts as low as parts per trillion can be separated and identified using HPLC. The primary benefits of HPLC are its high productivity in terms of cost parameters, great resolution, ease of use, generally high recoveries, and excellent reproducibility of repeat separations.